Fig. 2

Adipocytes decrease intracellular doxorubicin accumulation in breast cancer cells independently of major ABC transporters. a Representative experiments showing the intracellular accumulation of doxorubicin (DOX) detected by flow cytometry for indicated times and conditions. Histograms represent the quantification of DOX intracellular fluorescence for each cell line at indicated times after drug exposure. b Quantification of intracellular DOX at indicated times after drug exposure, in the presence (or not) of P-gp inhibitors: verapamil (Vp) and tariquidar (Tar) (left panel), detected by flow cytometry in MDA-MB436 and E0771 cells cocultivated (C) or not (NC) with adipocytes as indicated. Similar experiments were performed by using the MRP family inhibitor, MK-571 (MK) (middle panel), and the BCRP inhibitor, Fumitremorgin-C (FTC) (right panel). Abbreviation: a.u. arbitrary units.