Fig. 2

a Body weights were measured in MTB/TAN mice fed a high-fat diet (HFD) or low-fat diet (LFD) 4 weeks following tumor regression induced by doxycycline withdrawal. HFD mice weighed significantly more than LFD mice (32.9 ± 6.8 g vs. 25.2 ± 2.6 g, p < 0.001). b Representative photograph of mice fed a LFD or HFD. c HFD-Obese mice weighed more than HFD-Lean and LFD mice at enrollment (38.2 ± 5.1 g vs. 27.2 ± 1.8 g vs. 25.2 ± 2.6 g, respectively; p < 0.001; n = 50/arm). d HFD-Obese mice remained heavier than HFD-lean and LFD mice at study end (39.7 ± 5.4 g vs. 32.2 ± 3.9 g vs. 28.5 ± 3.9 g, respectively; p < 0.001; n = 50/arm). e Four weeks following doxycycline withdrawal, body fat composition determined by nuclear magnetic resonance was significantly increased in HFD-Obese mice compared with HFD-Lean and LFD mice, with greater total fat mass (14.3 ± 3.7 g vs. 6.9 ± 2.3 g vs. 4.2 ± 2.1 g, respectively; p < 0.001; n = 10/arm) and body fat percentage (38.6 ± 5.8% vs. 24.9 ± 6.0% vs. 15.7 ± 6.4%, respectively; p < 0.001). f HFD-Obese mice had greater body fat percentage than LFD and HFD-Lean mice at study end (36.8 ± 6.4% vs. 28.5 ± 6.7% vs. 22.4 ± 8.0%, p < 0.001; n = 50/arm). Figures are dot plots showing mean data. *p < 0.05 and ***p < 0.001