Fig. 1
From: Impact of obesity on breast cancer recurrence and minimal residual disease
Study design. Three-week-old female MTB/TAN mice were weaned onto matched study diets consisting of 60% calories from fat (high-fat diet) or 10% calories from fat (low-fat diet). Doxycycline (0.03 mg/ml) was provided in drinking water beginning at age 6 weeks to induce human epidermal growth factor receptor 2 (HER2)-driven mammary tumors. Once primary tumors reached target volume (500 cm3), the HER2 oncogene was downregulated by doxycycline withdrawal to induce tumor regression. Mice were then observed twice weekly for recurrence