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Table 1 Frequency of regeneration of gene-introduced mammary gland

From: A method of producing genetically manipulated mouse mammary gland

Mammary regeneration

First generation

Second generation

First experiment

7/18

11/16

Second experiment

3/18

12/14

  1. Mammary regeneration was judged from in-vivo imaging system analysis on the basis that > 1 × 106 photons/s of total flux is positive and partially from microscopic analysis on the basis of mCherry fluorescence. A fragment of primary mammary product (first generation) was dissected on day 85 (first experiment) or 105 (second experiment) after transplantation, cut into pieces, and then transplanted into the fat pad of newly prepared mice, and the secondary outgrowths (second generation) were allowed to expand for 75 (first experiment) and 28 days (second experiment)