Fig. 5

High loading capacity of the piggyBac donor vector for reconstitution of vector-introduced mammary glands. a Map of piggyBac transposon donor vector carrying long a DNA (approximately 200 kb) derived from a bacterial artificial chromosome (BAC) vector (RIKEN B6Ng01-263 N07) containing a fragmentary mouse genomic sequence encompassing at least eight known genes including Krt18. b Microscopic view of the BAC vector-introduced MECs. Red fluorescence (lower panels) shows mCherry marker driven by CAG promoter. Scale bar = 100 μm. c A representative image showing luciferase activity of mammary outgrowths at day 50 after mammary epithelial cell injection into cleared fat pad. d Microscopic view of resected fat pads from c. Red fluorescence (lower panels) shows mCherry marker expression. Scale bar = 5 mm