Fig. 5

ErbB2-induced Irf6 downregulation occurs in a mitogen-activated protein kinase-dependent manner. a MCF-ErbB2 cells were cultured for 24 h detached from the extracellular matrix (3D culture) in the presence of dimethyl sulfoxide or 1 μM SCH772984 and assayed for Irf6 expression by Western blotting. The membrane was reprobed with an anti-phospho-Rsk (pRsk) antibody and then an anti-Rsk antibody. b MCF10A cells and a variant of MCF10A cells obtained by infection of these cells with a retrovirus carrying constitutively active Mek2 mutant (MCF-MekDD) were kept in 3D culture for 3 h and assayed for Irf6 expression by Western blotting. β-actin was used as a loading control in (a) and (b)