Skip to main content
Fig. 4 | Breast Cancer Research

Fig. 4

From: ErbB2-driven downregulation of the transcription factor Irf6 in breast epithelial cells is required for their 3D growth

Fig. 4

Detachment-induced upregulation of Irf6 in nonmalignant breast epithelial cells requires the presence of ΔNp63α. a MCF10A cells were cultured attached to (2D culture) or detached from (3D culture) the extracellular matrix for the indicated times and assayed for ΔNp63 levels by Western blotting. b, c MCF10A cells transfected with 100 nM control RNA (cRNA) or p63-specific small interfering RNA (p63siRNA) 14 or 15 were kept in 3D culture for 3 h and assayed for ΔNp63 (b) or Irf6 (c) expression by western blot. β-actin was used as a loading control

Back to article page