Fig. 3

Intravital imaging of TDLN metastases reveals reduced migration of cancer cells in response to ezrin inhibition. a Maximum intensity projection (z-stacks of 4–6 μm) of still images from the time-lapse videos (see Additional file 6: video 3 and Additional file 7: video 4) showing migration of cancer cells (dotted line) in the inguinal LN. b Cell trajectory data generated for a minimum of 20 cells per group (3 fields per LN, 3 mice, pool of n=2 IVM studies; motile cells tracked by two observers blinded to the study). c The proportion of motile GFP-EO771LMV cells in TDLNs of control and NSC668394 treated mice (Mann Whitney test). d Mean displacement curves were generated from pooled cell trajectory data in panel B. The slopes of displacement curves (α-value) are shown beside each curve and compared using the Extra-Sum-of-Squares F test. e Overall mean velocities of motile cancer cells in control and treated TDLNs are shown (Mann Whitney test)