Alterations in arginine and energy metabolism, structural and signalling lipids in metastatic breast cancer in mice detected in plasma by targeted metabolomics and lipidomics

Table 1 Circulating white blood cell analysis^a

	1 week		2 weeks		3 weeks		4 weeks
	Control	4T1	Control	4T1	Control	4T1	Control	4T1
WBC^b	4.5 ± 1.1	4.3 ± 1.5	4.1 ± 0.7	12.8 ± 6.9	4.8 ± 0.8	50.6 ± 15.9	4.4 ± 1.1	189.0 ± 73.7
GRA^c	1.1 ± 0.2	1.4 ± 0.5	1.0 ± 0.3	8.1 ± 5.5	1.0 ± 0.2	35.7 ± 12.7	0.9 ± 0.3	143.6 ± 55.2
GRA%^d	23.6 ± 7.2	31.5 ± 6.8	21.1 ± 4.0	57.8 ± 11.6	18.6 ± 1.6	69.7 ± 4.9	18.4 ± 2.9	75.0 ± 3.6
LYM^c	3.3 ± 1.0	2.7 ± 1.0	3.0 ± 0.5	4.1 ± 1.2	3.7 ± 0.7	11.5 ± 2.8	3.3 ± 0.8	31.5 ± 11.9
LYM%^d	71.8 ± 8.9	63.5 ± 7.4	75.1 ± 4.5	36.9 ± 11.2	77.6 ± 1.6	23.5 ± 3.8	77.6 ± 3.0	17.2 ± 2.4
EOS^c	3.6 ± 1.4	5.0 ± 1.1	3.3 ± 1.1	11.4 ± 4.4	3.5 ± 0.6	9.2 ± 3.4	3.0 ± 1.7	66.5 ± 13.7
MON^c	0.14 ± 0.1	0.2 ± 0.1	0.1 ± 0.02	0.6 ± 0.3	0.1 ± 0.03	3.4 ± 1.4	0.1 ± 0.05	14.9 ± 8.3
MON%^d	4.6 ± 1.9	4.8 ± 1	3.8 ± 0.5	5.3 ± 1.1	3.8 ± 0.4	6.8 ± 1.8	3.9 ± 0.4	7.8 ± 1.8

Abbreviations: EOS Eosinophils, GRA Granulocytes, LYM Lymphocytes, MON Monocytes, WBC White blood cells
^aWhole blood was collected by cardiac puncture at the indicated time after orthotopic introduction of tumour cells, as described in the Methods section of text
^bTotal number of WBC per microliter of blood in thousands ± SEM
^cNumber of cells per microliter of blood in thousands ± SEM
^dThe percentage of total WBC ± SEM

ISSN: 1465-542X