Fig. 5

Fluvastatin treatment moderately inhibits growth of AT1-derived lesions. a Average weights of the MCF10.AT1 -driven xenografts from mice treated with fluvastatin or vehicle control. b Distribution of lesion grades within the xenografts, classified as histological grades 1 through 4, in control (n = 50 lesions) and fluvastatin-treated (n = 59) groups (n = 59 lesions) were similar in both groups (p = 0.68). c qPCR data showing the relative HMG-COA reductase (HMGCR) mRNA levels (normalized to ribosomal protein (RPL)19) in the lesions of vehicle-treated or fluvastatin-treated mice sub-grouped by histological grade after 16 weeks of treatment. Grade-0 samples, which had the lowest expression level (highest cycle threshold (Ct)) value were set as 1 and the differential mRNA expression for the rest of the samples was determined by calculating the fold change of mRNA above this lowest value using the Pffafl differential Ct method. Values represent mean fold change ± SEM