Fig. 4

Fluvastatin inhibits colonization ability and proliferation of breast preneoplastic cells. a Clonogenic survival assay showing crystal violet-stained colonies formed by preneoplastic MCF10.AT1 and MCF10.DCIS cells after 12 days of treatment with fluvastatin or vehicle control. b Quantification of colonies formed by MCF10.AT1 and MCF10.DCIS cells treated with fluvastatin or vehicle control. Values represent number of colonies (%) ± SEM. *p < 0.001. c, Dose-response curves of MCF10.AT1 and MCF10.DCIS cells showing significantly different half maximal inhibitory concentration values across these two cells lines (2.1 vs 18, *p < 0.01.), as measured by the MTT dye uptake method after 48 h of vehicle or fluvastatin treatment. Values represent cell survival (%) ± SEM