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Fig. 3 | Breast Cancer Research

Fig. 3

From: The isomiR-140-3p-regulated mevalonic acid pathway as a potential target for prevention of triple negative breast cancer

Fig. 3

Cholesterol pathway mediators HMG-COA reductase (HMGCR) and HMG-COA synthase1 (HMGCS1) are directly regulated by miR-140-3p-1. a qPCR results showing the effect of transfection of miR-140-3p-1 mimic on its predicted target genes relative to their expression, with scramble control mimic transfection that was set as 1. MCF10.AT1 cells were treated with scramble mimic and miR-140-3p-1 mimic for 48 h and the total cellular RNA was analyzed for expression of indicated mRNAs by qPCR. Values are normalized to RPL19 mRNA levels and change in target gene expression calculated under treatment conditions as fold change greater than the expression levels seen in control (mimic) treatment. b Schematic showing TargetScan-predicted binding sites of mature miR-140-3p-1 in the 3’ UTR of HMGCR or HMGCS1. c Luciferase assay showing normalized firefly expression in MCF10.AT1 cells 48 h after being transfected with the wild-type (WT) or mutated (MUT) HMGCR or HMGCS1 reporter along with the miR-140-3p-1/scramble mimic. A Renilla luciferase construct was co-transfected to control for transfection efficiency; the mean values shown are from three independent experiments. Values represent mean fold change ± SEM. *p < 0.05

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