Fig. 2

miR-140-3p-1 inhibits the colonization ability of breast preneoplastic cells. a Clonogenic survival assay showing reduction in the number of colonies formed by preneoplastic MCF10.AT1 and MCF10.DCIS cells with the transient transfection of a miR-140-3p-1 mimic relative to a scramble control mimic. b Quantification of percentage of inhibition in colonizing ability of AT1 and DCIS cells with miR-140-3p-1 transfection normalized to scramble control mimic transfection. c Transfection followed by qPCR assay showing the transfection efficiency of MCF10.AT1 and DCIS cells. Both the cell lines express mature miRNA-140-3p-1 more than 3500 times relative to scramble control mimic. Cells were treated with indicated mimics for 48 h and the total cellular RNA obtained was analyzed for expression of mature miR-140-3p-1 by qPCR. Values represent mean fold change ± SEM. *p < 0.05