Fig. 3

Reduced HER4 protein expression in siRNA-treated ZR-75-1 breast cancer cell line. Western blot analyses of HER4 (a), HER1 (b), HER2 (c), and HER3 (d) are shown for cell lysates gained from untreated ZR-75-1 control cells (–), cells treated with non-targeting siRNA (NT), and ZR-75-1 cells treated with targeting anti HER4/siRNA (T). One representative blot is shown, respectively, and repeated experiments (n = 3) are summarized in the corresponding bar charts. P values are calculated using one-way ANOVA and Tukey’s multiple comparisons test (****p < 0.0001). WT wild-type