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Fig. 1 | Breast Cancer Research

Fig. 1

From: Induction of cell cycle arrest and inflammatory genes by combined treatment with epigenetic, differentiating, and chemotherapeutic agents in triple-negative breast cancer

Fig. 1

ED regulates inflammatory, arrest, and differentiation genes. a Heatmap depicting unsupervised hierarchical clustering of top 10% differentially expressed genes in MDA-MB-231 cells following different treatments (2.5 μM entinostat, 1 μM ATRA, 0.2 μM doxorubicin). Color scale indicates log2 expression values. b Total number of genes regulated in MDA-MB-231 cells by indicated treatments. c Gene set variation analysis (GSVA) scores of gene set analysis (GSEA) hallmark gene sets for samples in the study. Heatmap colors from blue to red represent low to high enrichment; red color bar (right) shows false discovery rate (FDR)-adjusted p value from one-way analysis of variance (ANOVA) test comparing GSVA enrichment scores across treatment groups. d Volcano plot (log2 fold-change (FC) vs – log10 FDR) of genes upregulated and downregulated in MDA-MB-231 cells following treatment with ED in comparison to cells treated singly with E or D. Orange dots represent genes with at least 2-fold increase or decrease in gene expression and FDR < 0.05. e Heatmap of disease and biological functions analysis using Ingenuity® Pathway Analysis software on ED genes. Size and color of the boxes represent – log p value and z score, respectively. E entinostat (Ent), A all-trans retinoic acid (ATRA), D doxorubicin (Dox), Veh vehicle

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