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Fig. 3 | Breast Cancer Research

Fig. 3

From: Insulin-like growth factor receptor signaling in breast tumor epithelium protects cells from endoplasmic reticulum stress and regulates the tumor microenvironment

Fig. 3

Reduced insulin-like growth factor type1 receptor (IGF-1R) alters cytokine and chemokine production in tumor epithelia. a RT-PCR analysis of CCL2, IL-10, IL-6, and TNF-α in Wnt1/dnIGF-1R versus Wnt1 tumors (Student’s t test *P < 0.05, **P < 0.01; n = 5). b RT-PCR analysis of IL-6 in CD24+CD29lo, Lin− (CD45, CD31, Gr-1, Ter-119) luminal cells and CD24+CD29hi, Lin− (CD45, CD31, Gr-1, Ter-119) basal cells from Wnt1/dnIGF-1R versus Wnt1 tumors (Student’s t test *P < 0.05; n = 4, 3 technical replicates per sample). c RT-PCR analysis of IL-6 and CCL2 in human IgG (IgG) or IMC-A12 (A12) treated MCF7 cells (Student’s t test **P < 0.01, ***P < 0.001; n = 3, with three biological replicates per experiment). d RT-PCR analysis of IL-6 and CCL2 in MCF7 cells treated with IgG, A12, or A12 + N-acetyl-L-cysteine (NAC) (one-way analysis of variance compared to A12, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3, with three biological replicates per experiment)

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