Skip to main content
Fig. 6 | Breast Cancer Research

Fig. 6

From: Sulfatide decreases the resistance to stress-induced apoptosis and increases P-selectin-mediated adhesion: a two-edged sword in breast cancer progression

Fig. 6

Flow cytometry analysis of tumour cell–platelet aggregates. a MDA.C and (b) MDA.SUL cells labelled with DiD (red) and platelets labelled with DiO (green) incubated for 1 h at room temperature. Box marks mixed aggregates formed by cancer cells and platelets. Sulfatides present on breast cancer cells stimulate activation of platelets. Flow cytometric analysis of P-selectin expression using monoclonal antibody against P-selectin human platelets (106) (c) non-activated and activated with ADP at concentration of (d) 1 μM or (e) 5 μM incubated with (106) parental breast cancer MDA-MB-231 cells (solid line), control MDA.C cells (dotted line) and MDA.SUL with overexpression of sulfatide (grey). Platelets incubated only with secondary antibodies used as control (dashed line). ADP adensoine diphosphate

Back to article page