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Fig. 6 | Breast Cancer Research

Fig. 6

From: Sulfatide decreases the resistance to stress-induced apoptosis and increases P-selectin-mediated adhesion: a two-edged sword in breast cancer progression

Fig. 6

Flow cytometry analysis of tumour cell–platelet aggregates. a MDA.C and (b) MDA.SUL cells labelled with DiD (red) and platelets labelled with DiO (green) incubated for 1 h at room temperature. Box marks mixed aggregates formed by cancer cells and platelets. Sulfatides present on breast cancer cells stimulate activation of platelets. Flow cytometric analysis of P-selectin expression using monoclonal antibody against P-selectin human platelets (106) (c) non-activated and activated with ADP at concentration of (d) 1 μM or (e) 5 μM incubated with (106) parental breast cancer MDA-MB-231 cells (solid line), control MDA.C cells (dotted line) and MDA.SUL with overexpression of sulfatide (grey). Platelets incubated only with secondary antibodies used as control (dashed line). ADP adensoine diphosphate

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