Fig. 1From: Sulfatide decreases the resistance to stress-induced apoptosis and increases P-selectin-mediated adhesion: a two-edged sword in breast cancer progressionExpression of GAL3ST1 and sulfatide in breast cancer cell lines and characteristics of human breast cancer cell lines with overexpression or blocked expression of GAL3ST1 and sulfatide. a Expression of GAL3ST1 mRNA in breast cancer cell lines. Real-time PCR used to analyse GAL3ST1 mRNA. GAL3ST1 expression levels normalised against β-actin and MDA-MB-231 cells served as calibrator sample. Results expressed as mean. b Western blotting analysis of GAL3ST1 expression in breast cancer cell lines. Anti-GAL3ST1 rabbit polyclonal antibodies used to detect GAL3ST1 in cell lysates. c Immunostaining of gangliosides from human breast cancer cell lines, separated by HP-TLC, with mouse monoclonal antibody against sulfatide. d Western blotting analysis of GAL3ST1 expression in parental MDA-MB-231 cells, control MDA-MB-231 cells (MDA.CTR) and MDA-MB-231 cells overexpressing sulfatide (MDA.SUL). e Immunostaining of gangliosides from MDA-MB-231, MDA.CTR and MDA.SUL cells. f Western blotting analysis of GAL3ST1 expression in parental T47D cells, control T47D cells (T47D.CRISPR.C) and T47D cells with knockout of GAL3ST1 (T47D.Δ.GAL3ST1.1). g Immunostaining of gangliosides from T47D, T47D.CRISPR.C and T47D.Δ.GAL3ST1.1 cells. For western blotting, 40 μg of proteins separated by SDS-PAGE under reducing conditions on a 12% gel and electrophoretically transferred onto a nitrocellulose membrane. β-actin served as internal control. For immunostaining, aliquots of total gangliosides corresponding to 1 × 107 cells applied to HP-TLC plate. Gal3ST1 galactosylceramide sulfotransferaseBack to article page