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Fig. 1 | Breast Cancer Research

Fig. 1

From: Sulfatide decreases the resistance to stress-induced apoptosis and increases P-selectin-mediated adhesion: a two-edged sword in breast cancer progression

Fig. 1

Expression of GAL3ST1 and sulfatide in breast cancer cell lines and characteristics of human breast cancer cell lines with overexpression or blocked expression of GAL3ST1 and sulfatide. a Expression of GAL3ST1 mRNA in breast cancer cell lines. Real-time PCR used to analyse GAL3ST1 mRNA. GAL3ST1 expression levels normalised against β-actin and MDA-MB-231 cells served as calibrator sample. Results expressed as mean. b Western blotting analysis of GAL3ST1 expression in breast cancer cell lines. Anti-GAL3ST1 rabbit polyclonal antibodies used to detect GAL3ST1 in cell lysates. c Immunostaining of gangliosides from human breast cancer cell lines, separated by HP-TLC, with mouse monoclonal antibody against sulfatide. d Western blotting analysis of GAL3ST1 expression in parental MDA-MB-231 cells, control MDA-MB-231 cells (MDA.CTR) and MDA-MB-231 cells overexpressing sulfatide (MDA.SUL). e Immunostaining of gangliosides from MDA-MB-231, MDA.CTR and MDA.SUL cells. f Western blotting analysis of GAL3ST1 expression in parental T47D cells, control T47D cells (T47D.CRISPR.C) and T47D cells with knockout of GAL3ST1 (T47D.Δ.GAL3ST1.1). g Immunostaining of gangliosides from T47D, T47D.CRISPR.C and T47D.Δ.GAL3ST1.1 cells. For western blotting, 40 μg of proteins separated by SDS-PAGE under reducing conditions on a 12% gel and electrophoretically transferred onto a nitrocellulose membrane. β-actin served as internal control. For immunostaining, aliquots of total gangliosides corresponding to 1 × 107 cells applied to HP-TLC plate. Gal3ST1 galactosylceramide sulfotransferase

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