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Fig. 5 | Breast Cancer Research

Fig. 5

From: Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines

Fig. 5

Effect of granulocyte colony-stimulating factor (G-CSF) functional deletion on G-CSF secretion and primary tumor growth. Cells (5 × 105 4T1 and 4T1.G-CSF− cells) before and after colony selection were plated on a 6-well plate and the supernatant was collected after 24 h in culture. The experiment was repeated three times. a The concentration of G-CSF in the supernatant was detected by ELISA. Female balb/cByJ mice received subcutaneous injections of 5 × 105 4T1 (n = 5) or 4T1.GCSF− cells (n = 5). b Tumor volumes were recorded and blood was collected when tumors reached 500–700 mm3. Serum from naïve mice (n = 3) served as controls. c The concentration of G-CSF in serum was determined by ELISA (**p < 0.01 via one-way analysis of variance with Tukey’s post-hoc analysis)

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