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Fig. 3 | Breast Cancer Research

Fig. 3

From: Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines

Fig. 3

Cytokine release profile of 4T1 and EMT6 cells before and after irradiation. The 4T1 or EMT6 cells were exposed to 0 Gy (non-irradiated) or 100 Gy. The cells (5 × 105 non-irradiated (4T1 and EMT6) and irradiated (4T1 Irr and EMT6 Irr) cells) were seeded on separate T25 flasks and cultured for 48 h. Cell culture supernatants were collected from each flask and centrifuged to remove debris. Levels of IL-6 (a), granulocyte-macrophage colony-stimulating factor (GM-CSF) (b), monocyte chemotactic protein-1 (MCP-1) (c), and granulocyte colony-stimulating factor (G-CSF) (d) in cell-free supernatants were quantified using cytometric bead array. Levels of macrophage colony-stimulating factor (M-CSF) (e), vascular endothelial growth factor (VEGF) (f), and tumor growth factor-beta (TGF-β) (g) were quantified by ELISA. The experiment was repeated thrice and the results represent the mean ± standard error from one representative experiment (*p < 0.05, two-way analysis of variance with Tukey’s multiple comparisons post-hoc analysis)

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