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Fig. 1 | Breast Cancer Research

Fig. 1

From: Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines

Fig. 1

Effect of different doses of irradiation on proliferation of 4T1 and EMT6 cells. The 4T1 (a) and EMT6 (b) cells were irradiated at 0, 20, 40, 60, 80, or 100 Gy with respective cell culture medium and plated in a 96-well plate with 200 μl of fresh culture medium. After incubating at 37 °C for 24, 48, 72, and 96 h, 20 μl of CellTiter 96 Aqueous One Solution Reagent was added to each well and incubated for an additional 1 h. Number of viable cells in each well was then determined by measuring the absorbance at 490 nm and comparing it with a standard curve generated using known numbers of 4T1 or EMT6 cells. Results are represented as mean ± standard error (**p < 0.01, ***p < 0.001, ****p < 0.0001, one-way analysis of variance with Tukey’s post-hoc analysis)

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