Fig. 2
From: Disrupted circadian clocks and altered tissue mechanics in primary human breast tumours
Primary mammary epithelial cells (MECs) in 3D culture. a Phase contrast image of MECs isolated from normal and tumour breast tissue from the same patient, after 4 days in 3D culture (patient-4). MECs were plated at the same density but those isolated from tumour tissue formed larger clusters. The data are representative of similar studies from three patients. Scale bar 100 μm. b Left, representative Per2::Luc traces from cultures of MECs isolated from normal and tumour breast tissue (patient-3). Right, normalisation of Per2::Luc activity from normal and tumour MECs. Additional samples are shown in Additional file 4: Figure S4