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Fig. 2 | Breast Cancer Research

Fig. 2

From: BHLHE40 confers a pro-survival and pro-metastatic phenotype to breast cancer cells by modulating HBEGF secretion

Fig. 2

BHLHE40-knockdown (KD) reduced lung colonization of tumor cells inoculated into the circulation via tail veins. a Less tumor cells were detected in blood of NSG mice inoculated via tail vein injection with LM BHLHE40-KD than mice inoculated with empty vector (EV) control LM EV cells. Tumor cells in whole blood collected by cardiac puncture at indicated times after tail vein injection were isolated using the Ficoll-Paque PLUS medium and counted under fluorescent microscope. *p <  0.05 (n = 3, KD vs. EV), one-way ANOVA followed by Tukey’s post-hoc tests. b Fluorescent imaging of metastatic foci in lungs at different time points post tail-vein injection. c Percentage of areas occupied by metastatic loci in the lungs, as quantified by fluorescent imaging and ImageJ. *p <  0.05 (n = 3, KD vs. EV), one-way ANOVA followed by Tukey’s post-hoc tests

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