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Fig. 1 | Breast Cancer Research

Fig. 1

From: BHLHE40 confers a pro-survival and pro-metastatic phenotype to breast cancer cells by modulating HBEGF secretion

Fig. 1

BHLHE40-knockdown (KD) significantly reduced primary tumor size and lung metastatic burden in an orthotopic xenograft model. a BHLHE40-shRNA expression effectively reduced both baseline and hypoxia-induced expression of BHLHE40 protein in the LM cells, as determined by immunoblotting. b Orthotopic xenograft tumors derived from LM-BHLHE40-KD cells exhibited lower growth rate than tumors derived from control LM empty vector (EV) cells. NSG mice were inoculated in the inguinal mammary gland fat pads with 2 × 105 cells. Tumor size was monitored and measured weekly using a digital caliper. Tumor volume was calculated as: volume = (width2 × length)/2. *p <  0.05 (n = 20, KD vs. EV at indicated time points), one-way ANOVA followed by Tukey’s post-hoc tests. c Spontaneous lung metastasis detected by fluorescent imaging of lungs or human ALU repeats qPCR 5 weeks after inoculation of tumor cells in mammary gland fat pads. *p <  0.05 (n = 10, KD vs. EV), Student’s t test. d Lung metastasis in mice after resection of primary tumors. Primary tumors in mammary gland fat pads were resected when they reached a size of 5 × 5 mm and lung metastasis were analyzed 4 weeks post-resection by fluorescent imaging of lungs or human ALU repeats qPCR. *p <  0.05 (n = 10, KD vs. EV), Student’s t test

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