Fig. 2

Bioprinting of as few as 10 cells per print location results in consistent organoid formation. MCF12A cells were printed using initial cell concentration of 5, 10, 40, or 60 cells (columns left to right, respectively) at a distance of 500 μm between print locations. Images were taken at days 4, 5, 6, 7, 9, 10, 12, and 14 (rows top to bottom, respectively). Initial injections of 10 or more cells resulted in consistent organoid formation. Consistent fusion of multiple print locations was seen by day 14 when at least 10 cells were printed per injection site. Scale bar: 500 μm