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Fig. 3 | Breast Cancer Research

Fig. 3

From: Efficient and tumor-specific knockdown of MTDH gene attenuates paclitaxel resistance of breast cancer cells both in vivo and in vitro

Fig. 3

Effect of metadherin (MTDH) expression on the efficacy of paclitaxel (TAX) in MCF-7 cells. a Cytotoxicity was evaluated by Cell Counting Kit-8 assay after treatment with TAX for 48 h. Abbreviation: OD optical density. b The inhibition rates of MCF-7–MTDH–short hairpin RNA (MCF-7–MTDH–shRNA) cells were much higher than those of MCF-7–MTDH cells. c MTDH suppressed cell apoptosis induced by TAX, while MTDH silencing increased the sensitivity of TAX. d Knockdown of MTDH increased the G2/M phase arrest induced by TAX, while the opposite effect was found in MTDH overexpressing MCF-7 cells. e Western blot analysis revealed that protein expression of p65 was higher in MCF-7–MTDH cells, while IκBα was lower. On the contrary, in MCF-7–MTDH–shRNA cells, the protein expression level of p65 decreased and IκBα increased. Knockdown of MTDH could inhibit the activity of the nuclear factor-kappa B (NF-κB) pathway. *P <0.05 and **P <0.01 compared with MCF-7

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