Fig. 4

Cholesterol synthesis regulator, sterol regulatory element-binding protein 1 (SREBP1) is upregulated in long-term estrogen deprivation (LTED) cells. a Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of SREBP1a in LTED and parental cells. Parental cells were cultured in their normal growth media (fetal bovine serum, or FBS). Plots are the combination of data from three independent experiments. Data are mean ± standard deviation (SD) of nine replicates. One-way analysis of variance (ANOVA) followed by Dunnett’s post hoc test for multiple comparisons, *P <0.05, **P <0.01, ***P <0.001. b The expression of precursor SREBP1 (pre-SREBP1) and mature SREBP1 (mSREBP1) in parental and LTED cells. Because of their similar molecular weight (54 amino acid difference in length), SREBP1a and SREBP1c were not separated in the gel. β-actin and PCNA were used as internal control in the whole cell lysis and nuclear lysis, respectively. The band of mSRBP1 is labeled with an arrow. Blots of whole cell lysis are representative of three independent experiments, and the blot of nuclear lysis is a single experiment. c RNA and protein expression levels of fatty acid synthase (FASN). Plots are representative of two independent experiments. Data in the left panel are mean ± SD of three replicates. One-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons, *P <0.05, **P <0.01, ***P <0.001. d The growth inhibition of MM134 parental and LTED cells by etomoxir. MM134 LTED-D was selected as the representative variant of MM134 LTED cells. This figure is the same experiment as the dose response curve of etomoxir in Additional file 1: Figure S8B. Plot is representative of two independent experiments. Data are mean ± SD of six replicates. One-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons, ***P <0.001