Fig. 7
Analysis of A375 melanoma cell line xenograft growth. Human melanoma cells (A375; 5 × 105) were injected orthotopically into the skin of both nonhumanized nonobese diabetic/severe combined immunodeficiency IL2Rγnull (NSG) and humanized NSG (hNSG) mice, after which (initial tumor volume 150–200 mm3) they were randomly sorted into treatment groups. Non-hNSG mice (a) or hNSG mice (b and c) were treated weekly with vehicle (control), anti-CTL4 (2.5/5 mg/kg (b), or anti-PD-1 (10 mg/kg) (c) antibodies. Tumor growth was evaluated twice weekly. If tumor volume reached 1500–2000 mm3, mice were killed as per humane animal welfare regulations. *P < 0.05, **P < 0.01, *** P < 0.001. NS Nonsignificant