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Fig. 1 | Breast Cancer Research

Fig. 1

From: Evaluation of anti-PD-1-based therapy against triple-negative breast cancer patient-derived xenograft tumors engrafted in humanized mouse models

Fig. 1

Analysis of human immune cell engraftment. a Evolution of the percentage of human CD45+ cells after intravenous (i.v.) injection of hCD34+ hematopoietic stem cells. Cells were identified by flow cytometry in circulating blood collected from humanized mice at the indicated time intervals (n = 8). b Analysis of hCD45+ and corresponding subpopulations, including hCD20+ (B cells), hCD3+ (T cells), hCD33+ (myeloid lineage), hCD56+ (natural killer [NK] cells), and hCD68+ (macrophages) cells, was determined by flow cytometry in blood, bone marrow, and spleen samples collected from humanized nonobese diabetic/severe combined immunodeficiency IL2Rγnull (hNSG) mice after 22 weeks of i.v. injection of human hematopoietic stem cells (n = 8). c Representative IHC analysis of human CD45+, CD20+, CD68+, and CD56+ cells performed in preparations of spleen from humanized (upper row) and nonhumanized (lower row) NSG mice. Counterstain, hematoxylin; magnifications, 20× and 4× (inset)

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