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Fig. 5 | Breast Cancer Research

Fig. 5

From: Mucosal associated invariant T cells from human breast ducts mediate a Th17-skewed response to bacterially exposed breast carcinoma cells

Fig. 5

a Cells isolated from primary human breast ducts were exposed to MDA-MB-231 breast carcinoma cells that were mock-treated or pulsed with fixed Escherichia coli in the presence or absence of an anti-MR1 blocking antibody. Left plots: Flow cytometric analysis of intracellular interferon (IFN)-γ and interleukin (IL)-17A staining by the mucosal associated invariant T (MAIT) cell population from one representative experiment. Graphs on right: Intracellular cytokine analysis results from three independent experiments. b In vitro-expanded MAIT cells derived from breast duct (top row) or from peripheral blood mononuclear cells (PBMCs) (bottom row) were stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin (left column) or exposed to E. coli-pulsed MDA-MB-231 cells (right column). Expression of IFN-γ and IL-17A was assessed by intracellular cytokine staining. APCs Antigen-presenting cells

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