Fig. 3
From: Obesity promotes the expansion of metastasis-initiating cells in breast cancer
Increased tumor hypoxia in obese mice. Tumors in mice fed a high-fat diet (HFD) have higher Ki67+ counts (a, scalebar 200 um). CD31 immunohistochemical analysis shows lower vessel density in tumors from HFD mice (b, scalebar 200 uM). This is supported by fluorescence-activated cell sorting quantification (c). Pimonidazole (PIMO) staining in mouse fed a regular diet (RD) or a HFD demonstrate greater hypoxic areas in tumors from HFD mice (d, scalebar 200 uM). Quantitative PCR analyses on RD and HFD tumors show upregulation of hypoxia inducible factor 1 alpha (HIF1α) targets (e, n = 5) and faster tumor progression (f, n = 5). Arbp, attachment region binding protein