Fig. 1

Effects of a high-fat diet (HFD) on tumor progression in mice. The tumor weight is increased in the HFD groups in both EO771 grafts (a, n = 14 regular diet (RD) and n = 16 HFD) and Py230 grafts (b, n = 11 RD and n = 15 HFD). Tumor weight correlates with body weight (c, N = 60). The number of lung metastases is increased in both EO771 tumor-bearing mice (d, n = 14 RD and n = 16 HFD) and Py230 bearing mice (e; n = 19 sections, RD and n = 61 sections, HFD). Py230 cells have the same extravasation capabilities in both obese and lean mice as seen by the percentage of Celltracker-labeled cells in the lungs at 2 h (n = 3 RD and n = 3 HFD) and 48 h (n = 4 RD and n = 4 HFD) by FACS (f). The number of metastatic colonies is also not changed in the RD compared to the HFD groups in Py230 (g, n = 4) or E0771 (h, N = 11)