Fig. 5

Nogo-B receptor (NgBR) regulates protein levels of p53 and survivin through Ras-mediated pathways. a Knockdown of both H-Ras and K-Ras increases the protein levels of p53 and decreases the protein level of survivin. Control siRNA and siRNAs targeting either H-Ras or K-Ras siRNA were transfected into MCF-7-TamR cells. b Overexpression of either NgBR-HA or HER2-HA in MCF-7 cells decreases the protein levels of p53, ERα, and increases the protein level of survivin. Knockdown NgBR in MCF-7 cells overexpressing HER2-HA restores the protein levels of p53, ERα and survivin to levels similar to those in control MCF-7 cells transfected with empty plasmid DNA vector. Change in NgBR or human epidermal growth factor receptor 2 (HER2) has no effects on the total protein levels of AKT and extracellular signal-related kinase (ERK)