Fig. 7

TNFAIP3 KO inhibits DCIS-iFGFR1 cell-derived xenograft tumor growth and progression in mice. a Tumor growth curves. Two million DCIS-iFGFR1 or TNFAIP3 KO DCIS-iFGFR1 cells were injected into one of the fourth pair mammary gland fat pads of nude mice on day 1. Six to ten mice in each group were treated with vehicle (V) or AP20187 (AP) as indicated from day 4 to day 14. Tumor volume was measured as described in the Methods section and presented as mean ± SD. * and **p < 0.05 and p < 0.01. b Images of individual tumors derived from the indicated cells in mice treated with vehicle or AP20187 as indicated. c Average weights of wet tumors collected from mice shown in Panel A on day 14. The number of tumors weighed in each group is indicated. ***p < 0.001 by unpaired Student’s t test. d Immunohistochemical staining for p-ERK1/2 (brown color) in the tissue sections prepared from vehicle or AP20187-treated DCIS-iFGFR1 control and TNFAIP3 KO xenograft tumors. e H&E-stained tissue sections prepared from vehicle- or AP20187-treated DCIS-iFGFR1 control and TNFAIP3 KO xenograft tumors. The boxed areas are also shown in higher magnification as indicated. DCIS ductal carcinoma in situ-like area, IC invasive carcinoma-like area, M skeletal muscle area, SL surrounding stromal cell layer