Fig. 6

In vivo effects of human epidermal growth factor receptor 3 (HER3) vaccine-induced antibody (HER3-VIA) in triple negative MDA-MB-468 SCID tumor xenografts. a Flow cytometry analysis of HER family expression by MDA-MB-468 cells. Cells were stained with phycoerythrin (PE)-conjugated anti-epidermal growth factor receptor (anti-EGFR), anti-HER2, or anti-HER3 monoclonal antibodies (mAb) (upper three histograms), or were incubated with HER3-VIA or green fluorescence protein (GFP)-VIA (1:100 dilution), followed by PE-conjugated anti-mouse IgG (lower two histograms). b Effect of HER3-VIA on signaling pathway was analyzed by western blotting. MDA-MB-468 cells were incubated with HER3-VIA (1:100 dilution) in vitro at 37 °C for the indicated time period. c Passive transfer of HER3-VIA retarded the growth of established MDA-MB-4684 in SCID mice: *p < 0.001). d Western blot analysis of the in vivo signaling effects of HER3-VIA exposure compared to control and GFP-VIA in triple negative MDA-MB-4684 SCID tumor xenografts. GAPDH, glyceraldehyde-3-phosphate dehydrogenase