Fig. 9
From: NOTCH3 expression is linked to breast cancer seeding and distant metastasis
Self-renewal and invasive capacity of patient-derived triple-negative breast cancer (TNBC) cells. a Immunoblot assay showing total Aurora kinase A (AURKA), phosphorylated AURKA (p~AURKA), and NOTCH3 expression in MDA-MB-231 and patient-derived TNBC-M25 cells. b Densitometry analysis showing the percentage of p~AURKA and NOTCH3 protein levels in TNBC-M25 cells relative to MDA-MB-231 cells. Graph shows the average from three independent experiments (± SD). c Representative images of light microscopic analysis showing single-cell dilution tertiary mammosphere (MPS) from TNBC-M25 cells infected with scramble lentiviral short hairpin RNAs (lenti-shRNAs; control) and lenti-shRNAs targeting NOTCH3 messenger RNA (mRNA). d Graphs showing the average size from three independent experiments (± SD) of tertiary MPS derived from TNBC-M25 cells infected with scramble lenti-shRNAs (control) and lenti-shRNAs targeting NOTCH3 mRNA. MPS size was quantified using the National Institutes of Health ImageJ software (http://imagej.nih.gov/ij). e In vitro real-time invasion assay of TNBC-M25 cells infected with scramble lenti-shRNAs (control) and lenti-shRNAs targeting NOTCH3 mRNA. f Graph showing the average number of invasive cells from three independent experiments (± SD)