Fig. 6
From: NOTCH3 expression is linked to breast cancer seeding and distant metastasis
Invasive capacity of vMCF-7∆Raf1 1GX cells with abrogated NOTCH3 expression. a In vitro real-time invasion assay of vMCF-7∆Raf1 1GX and vMCF-7∆Raf1 1GX/CRISPR-NOTCH3 cells stained in red with 5 μM Cell Tracker Red CMTPX. b Graph showing the average number of invasive cells from three independent experiments (± SD). c Immunoblot assay showing NOTCH3 expression in vMCF-7∆Raf1 1GX cells infected with scramble lentivirus short hairpin RNA (lenti-shRNA; control) and lenti-shRNAs targeting NOTCH3 messenger RNA (mRNA). d Densitometric analysis showing the percentage of NOTCH3 protein level in vMCF-7∆Raf1 1GX/shRNA-NOTCH3 cells relative to control. Graph showing the average from three independent experiments (± SD). e In vitro real-time invasion assay of vMCF-7∆Raf1 1GX cells infected with scramble lenti-shRNAs (control) and lenti-shRNAs targeting NOTCH3 mRNA. f Graph showing the average number of invasive cells from three independent experiments (± SD)