Fig. 1
From: NOTCH3 expression is linked to breast cancer seeding and distant metastasis
Establishment of metastatic breast cancer xenografts. a Lungs isolated from nude mice carrying MCF-7 and vMCF-7∆Raf1 tumor xenografts. Following 12 weeks of growth, animals were killed, and lung tissue was stained with H&E to determine the presence of metastatic nodules. b Immunofluorescence assay showing representative images of centrioles and pericentriolar material (PCM) in MCF-7 and variant breast cancer cells. Centrioles were labeled in green with monoclonal 20 h5 centrin antibody, and PCM was labeled in red with polyclonal pericentrin antibody. c Graph showing the average percentage of cells with more than four centrioles from three independent experiments (± SD). HMEC Human mammary epithelial cells