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Fig. 5 | Breast Cancer Research

Fig. 5

From: Tricho-rhino-phalangeal syndrome 1 protein functions as a scaffold required for ubiquitin-specific protease 4-directed histone deacetylase 2 de-ubiquitination and tumor growth

Fig. 5

Transcription output analysis of the tricho-rhino-phalangeal syndrome 1 (TRPSI)-ubiquitin-specific protease 4 (USP4)-histone deacetylase 2 (HDAC2) regulatory axis. a Analysis and comparison of transcriptomes of MCF7 upon silencing TRPS1 or HDAC2 using RNA sequencing. b Validation of the selected TRPS1 target genes using RT-qPCR. c Validation the selected HDAC2 target genes using RT-qPCR. d Expression levels of genes up-regulated upon silencing of TRPS1 were restored with overexpression of HDAC2. e Selected genes upon silencing of TRPS1 or HDAC2 show consistent up-regulation by RT-qPCR upon silencing of USP4 in MCF7. f Expression levels of genes up-regulated upon silencing of USP4 were restored with additional overexpression of HDAC2. g, h Chromatin immunoprecipitation (ChIP)-qPCR using H4K16AC antibodies on selected target genes upon silencing of TRPS1 or USP4 in MCF7 cells. The t test was used for statistical quantifications: *p < 0.05, **p < 0.01, ***p < 0.001, respectively. SiRNA, small interfering RNA

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