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Table 1 Lung/spleen weight and blood count in the orthotopic 4T1 breast cancer model in mice

From: Nitric oxide deficiency and endothelial–mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice

Parameter Week after 4T1 cancer cell inoculation
Control 1st 2nd 3rd 4th 5th
Lung weight (g) 0.76; 0.70–0.80 (n = 30) 0.77; 0.70–0.81 (n = 29) 0.76; 0.72–0.84 (n = 30) 0.81; 0.76–0.85 (n = 29) 0.0.82; 0.77–0.91 (n = 30) 1.7; 1.3–2.22*** (n = 49)
WBC (K/μl) 4.20; 3.41–5.22 (n = 35) 4.70; 4.04–5.28 (n = 21) 5.55; 4.94–6.21 (n = 23) 17.02; 11.85–24.78*** (n = 23) 54.62; 96.18–177.10*** (n = 20) 257.30; 148.20–317.10*** (n = 36)
GRA (K/μl) 1.00; 0.80–1.20 (n = 35) 1.25; 1.10–1.40 (n = 21) 1.85; 1.35–2.40* (n = 23) 11.30; 6.95–16.10*** (n = 23) 79.00; 42.48–132.80*** (n = 20) 159.80; 89.25–220.90*** (n = 36)
LYM (K/μl) 3.10; 2.50–3.85 (n = 35) 3.25; 2.75–3.87 (n = 21) 3.40; 2.80–3.90 (n = 23) 5.65; 4.15–7.00*** (n = 23) 18.50; 9.75–24.13*** (n = 20) 46.90; 25.00–95.00*** (n = 35)
Spleen weight (g) 0.10; 0.09–0.11 (n = 20) 0.10; 0.09–0.11 (n = 30) 0.12; 0.11–0.13 (n = 30) 0.24; 0.20–0.32** (n = 30) 0.54; 0.43–0.68*** (n = 30) 0.89; 0.79–0.99*** (n = 70)
  1. Data presented as median; interquartile range. Blood count performed to assess development of systemic inflammation whereas spleen weight recorded as an indirect marker of systemic inflammation [48]. Based on normality of distribution and variance homogeneity, data were analyzed with Kruskal–Wallis test followed by Dunn’s multiple comparison test. Statistical significance vs healthy control mice at *P < 0.05, **P < 0.01 and ***P < 0.001
  2. WBC white blood cells, GRA granulocytes, LYM lymphocytes