Fig. 7
From: Pyruvate carboxylase supports the pulmonary tropism of metastatic breast cancer
PC supports metabolic flexibility and oxidative stress defense. a Glycolytic stress test analysis of 4 T07 and 4 T1 control (shscram) and PC-depleted (shPC25) cells. b ECAR measurements obtained upon injections of glucose (10 mM), oligomycin (Oligo) and 2-deoxglucose (2-DG) used to calculate glycolysis and glycolytic capacity in each cell line. c OCR measurements for 4 T07 and 4 T1 control (shscram) and PC depleted (shPC25) cells in the presence of 10 mM glucose, 2 mM glutamine and 1 mM pyruvate. Data are the mean ± SE of measurements per μg protein. d Control (shscram) and PC-depleted (shPC25 and shPC28) 4 T1 cells were treated with the indicated concentrations of H2O2 and viable cells were visualized with crystal violet. e Stained cells from panel D were solubilized and quantified via absorbance at 600 nm. Data are the mean ± SD of three independent experiments. Asterisks indicates statistical significance (*P < 0.05, ***P < 0.01). ECAR extracellular acidification rate, OCR oxygen consumption rate