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Fig. 6 | Breast Cancer Research

Fig. 6

From: Pyruvate carboxylase supports the pulmonary tropism of metastatic breast cancer

Fig. 6

PC is not required for the growth of extrapulmonary metastases. a Immunoblot analysis for PC in 4 T07 cells expressing control (scram) or PC-targeted (shPC25) shRNAs. Analysis of β-tubulin (β-tub) served as a loading control. b 4 T07 cells (5.0 × 105/mouse) were injected into the lateral tail vein of female Balb/c mice. Pulmonary tumor cell delivery (Day 0) and tumor formation (Day 21) were visualized by bioluminescent imaging. c Bioluminescent intensity (Radiance) values were normalized to the injected values for each group and used to quantify thoracic tumor growth in intact mice 21 days after tumor cell injections. Data are the mean normalized values ±SE of five mice per group resulting in the indicated P value. d Necropsy pictures showing representative lungs of mice 21 days after tail vein injections of control (scram) and PC depleted (shPC25) 4 T07 cells. e The numbers of pulmonary tumor nodules per lobe was quantified in mice injected with control (scram) and PC-depleted (shPC25) 4 T07 cells. Data are the mean ± SE of five mice per group resulting in the indicated P value. f The lungs of mice injected with control (scram) and PC-depleted (shPC25) 4 T07 cells were removed upon necropsy and the carcasses were immediately imaged. Shown are representative images from each group. g Whole animal bioluminescent intensity measurements were taken for dissected carcasses described in panel F. Data are the mean radiance values ±SE of five mice per group resulting in the indicated P value. PC pyruvate carboxylase

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