Fig. 4
From: Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery
Multi-modal spectral histopathology (MSH) diagnosis generated using auto-fluorescence (AF) and raster scan Raman measurements of breast samples. Diagnosis for Raman raster scan is presented as tumor probability (P) (output of the classification model), while the diagnosis of each segment in the MSH is presented as tumor score (TS). Segmentation and sampling algorithms use AF images to focus Raman measurements (red circles) to suspicious regions, greatly reducing the number of spectra required for accurate diagnosis. Areas detected as tumor in the first round of MSH measurements are sampled by further Raman measurements (magenta crosses). a) Invasive carcinoma (IC); b) lobular carcinoma in situ (LCIS); c) ductal carcinoma in situ (DCIS)