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Fig. 1 | Breast Cancer Research

Fig. 1

From: Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery

Fig. 1

Instrument and procedure for multimodal spectral histopathology (MSH). a The MSH instrument consists of an inverted optical microscope with integrated Raman spectrometer (excitation 785 nm, detection Raman shift range 600–1800 cm− 1) and confocal auto-fluorescence (AF) module (excitation 405 nm, detection range 450–520 nm). b The MSH measurement procedure can be completed in 12–24 min, depending on tissue size (up to 4 × 6.5 cm2). Steps in white boxes are automated (do not require user input). After MSH analysis, the tissue is returned for normal histopathology analysis

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