Fig. 4
From: Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases
Characterization of the metastatic microenvironment and metastatic carcinoma cell proliferation/death. Lung metastases arising in Sdc1 +/+ and Sdc1−/− mice after tail vein injection of 4T1 cells were analyzed by immunofluorescence or second harmonic generation microscopy and the signal was quantified using ImageJ. a Leukocyte marker CD45 (red) b Proliferation marker Ki67 (green). c Apoptosis marker active caspase 3 (aCasp 3) (green). Original magnification approximately × 400 for all images. Metastatic lesions are outlined with dashed lines. Abbreviations: M, metastasis; L, lung parenchyma; DAPI, 4′,6-diamidino-2-phenylindole