Fig. 2

Effect of host syndecan-1 (Sdc1) on later stages of the metastatic cascade. The 4T1 tumor cells (1 × 10⁵ tumor cells in 100 μL serum-free DMEM) were injected into the tail vein and mice were sacrificed 15 days later. a Small, early metastasis, with carcinoma cells invading through lung vessel wall and surrounding vessel (M, metastasis; V, vessel; Br, bronchiole; original magnification × 400; scale bar indicates 100 μm). b Number of metastatic lesions per mouse. Metastases were counted on single histologic sections of both lungs. c Metastatic tumor burden expressed as percent lung tissue occupied by metastatic lesions. d Average area of metastatic lesions expressed in pixels as measured on histologic sections