Fig. 3From: Evaluation of α-tubulin, detyrosinated α-tubulin, and vimentin in CTCs: identification of the interaction between CTCs and blood cells through cytoskeletal elementsExpression of cytokeratin (CK), vimentin (VIM), and detyrosinated α-tubulin (GLU) in patients’ circulating tumor cells (CTCs). Patients’ samples were stained with pancytokeratin (A45-B/B3, green), detyrosinated tubulin (blue), and vimentin antibodies (red) and 4′,6-diamidino-2-phenylindole (DAPI, not shown). a–d Representative confocal laser scanning micrographs of patients’ CTCs (× 60) stained with pancytokeratin (A45-B/B3), vimentin, and GLU antibodies. Intercellular connections through cytoskeletal bridges (white arrows) were observed between CTCs. These microtentacles were supported by GLU, VIM, and CK. e–h CK, VIM, and GLU expression on a patient’s CTC (× 60), which is in contact with a peripheral blood mononuclear cell from a patient sampleBack to article page