Fig. 4
From: ETV4 transcription factor and MMP13 metalloprotease are interplaying actors of breast tumorigenesis
MMP13 acts as ETV4 in the modification of tumorigenic properties of MMT cells. a and d MMT-Ctrl and MMT-MMP13 (a) or MMT-shCtrl and MMT-shMMP13 (d) cell proliferation analysis by cell counting. The two charts represent the number of counted cells at 10, 35, 55, 80, and 100 hours. Experiments were conducted three times in triplicate. Error bars indicate SD. ****P ≤ 0.0001; ***P ≤ 0.001; *P ≤ 0.1. b and e MMT-Ctrl and MMT-MMP13 (b) or MMT-shCtrl and MMT-shMMP13 (e) cell migration analysis using a Boyden chamber culture system. Histogram represents the relative number of counted cells that migrated to the lower side. The number of MMT-Ctrl (b) and MMT-shCtrl (e) cells was arbitrarily = 1. Experiments were conducted three times in triplicate. Error bars indicate SD. ****P ≤ 0.0001. The lower panel depicts a representative picture of each experiment. Scale bar = 100 μm. c and f Anchorage-independent growth. MMT-Ctrl and MMT-MMP13 (c) or MMT-shCtrl and MMT-shMMP13 (f) cells were cultured for 10 days in soft agar. This histogram represents the number of clones counted for experimental time points. Soft agar assays were conducted three times in triplicate. Magnification × 5. Error bars indicate SD. ****P ≤ 0.0001; **P ≤ 0.01. The lower panel depicts a representative picture of each experiment. Scale bar = 100 μm