Fig. 1

Trastuzumab induces anti-HER2 antibodies during adjuvant treatment of resected HER2+ BC patients. a shows the median time (gray bar) at which samples were collected post-treatment for each arm, calculated from the inset scatter dots each of which represents a unique sample from a unique patient. Inset colored bars show treatment timeline for each arm with the red bar representing adriamycin and cyclophosphamide (AC) therapy, the green bar paclitaxel, and the blue bar trastuzumab. P value was calculated using one-way ANOVA. b shows Kaplan-Meier analysis comparing disease-free survival (DFS) for each of the cohorts of patients from which samples were drawn. c-e show the individual changes in antibody levels given indicated as the relative index (RI) for Arms A-C of N9831, respectively. Each line is calculated from triplicate measurements for each pre- and post-treatment specimen. The inset blue dotted line represents the cutoff for what is considered a positive antibody level (see Materials and Methods). P values were calculated using the paired Student’s t test