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Fig. 5 | Breast Cancer Research

Fig. 5

From: NDRG1 regulates neutral lipid metabolism in breast cancer cells

Fig. 5

NDRG1 depletion increases neutral lipid storage in lipid droplets. a Boron-dipyrromethene (BODIPY) 493/503 and Hoechst-stained stable SKBR3 cells expressing NDRG1 or green fluorescent protein (GFP) targeting shRNA at 2 weeks post selection. Scale bar = 25 μm. b Comparison of BODIPY-positive lipid droplet specific signal/cell in stable SKBR3, MCF7 1% O2, MDA-MB-231, MDA-MB-468, and HCC1569 expressing the indicated shRNAs: all N = 6 and ***p < 0.00001. See Additional file 1: Figure S6 for related data. c Comparison of lipid droplet signal in stable SKBR3 cells expressing FLAG tagged full-length NDRG1 and N and C terminal truncations as indicated. N = 12, **p < 0.01, ***p < 0.00000001, scale bar = 25 μm. d Electron microscopy analysis of Hs578T cells +/− NDRG1 depletion. Arrows indicate lipid droplets. Scale bar = 0.5 μm. e-f Analysis of thapsigargin and tunicamycin and survival by counting Hoechst-stained nuclei 48 h post stable SKBR3 +/− NDRG1 depletion. Plots represent surviving cell fraction compared to untreated cells, N = 3. All bar graphs represent mean +/− SD and comparisons were by the two-sided t test. Scale bars = 25 μm

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